Ic50 graphpad prism1/7/2023 If I am comparing IC50 values for viability honestly I do it manually by reading of the IC50 value for each individual experiment (not replicate just the average for each experiment). The problem with calculating IC50 values in Prism is that you must have proper dose response curves for it to work properly as in calculates based on a formula (hope I am making sense ) i.e if you viability curve does reach 0% it can cause problems. These results drastically change fold changes. I tried GraphPad Prism to calculate ic50, however it gives different results from the results of excel.
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